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. 2017 Mar 30;12(3):e0174742. doi: 10.1371/journal.pone.0174742

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© 2017 Azumaya et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — (A1) Normalized fluorescence data for VSD assay on A2R-stg cells with 50 μM fluorowillardiine (black) with maxGLU (red) and NBQX (pink) controls, all normalized to EC₅₀GLU (blue). (A2) CRC curves for FW against A2-stg and (A3) A2-C3 cell lines calculated from the CMPDslope window. %max GLUslope = (CMPDslope—mean VHLslope)/(mean maxGLUslope—mean VHLslope) is further described in methods. (B1) Normalized fluorescence data for VSD assay on A2R-stg cells with 1 mM CX-546 (black) with maxGLU (red) and NBQX (pink) controls, all normalized to EC₅₀GLU (blue). (B2) CRC curves for CX-546 against A2R-stg and (B3) A2R-C3 cell lines calculated from the GLUslope window. %max GLUslope = (GLUslope—mean VHLslope)/(mean maxGLUslope—mean VHLslope) (C1) Normalized fluorescence data for VSD assay on A2R-stg cells with 500 μM cyclothiazide (CTZ, black) with maxGLU (red) and NBQX (pink), all normalized to EC₅₀GLU (blue). (C2) CRC curves for CTZ against A2-stg and (C3) A2-C3 cell lines calculated from the GLUslope window. Compound EC₅₀ values that could be reliably calculated are in the top left corner of each graph.