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. 2017 Mar 30;12(3):e0174742. doi: 10.1371/journal.pone.0174742

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© 2017 Azumaya et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 7 — (A) Raw data for compound CRCs in the VSD assay. A2R-stg (orange) and A2R-C3 (blue). EC₅₀GLU traces are represented by dashed lines. Compound concentrations are indicated in the top left corner. (B) CRCs calculated from GLUslope in the VSD assay for A2R-stg and A2R-C3 cells. Error bars are standard deviations. (C) Raw data for compound CRCs in the calcium flux assay. A2Q (red), A2Q-stg (orange), A2Q-GSG (green), and A2Q-C3 (blue). Dashed lines are signal of 1mM glutamate without compound. (D) Compound CRCs in calcium flux assay for A2Q-stg, A2Q-C3, A2Q-GSG, and A2Q cell lines. These are derived from the GLUslope1 window. (E) Compound CRCs calculated from the AUC in the GLUmaxmin window of the calcium flux assay plotted as %max AUC in the GLUmaxmin window (see Fig 4C) vs. log [compound].