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. 2017 Mar 30;12(3):e0174804. doi: 10.1371/journal.pone.0174804

Fig 4. Cleavage efficiency analyses of different vector transformed protoplasts.

Fig 4

(A) Western blot analysis of cleavage efficiency of the linker peptide in protoplasts. The transformed protoplasts were processed for Western blot 24-h post-transformation. The cleavage efficiency was assessed using GFP, GUS and 2A antibodies. Anti-β-actin antibody was used as a loading control. Asterisks represent uncleaved proteins. (B) Quantification of the cleavage efficiency of different linker peptides. Cleavage efficiency = cleaved form/(cleaved form + uncleaved form). The amount of each form was estimated from its band intensity on the western blot measured by Image J software.