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. 2017 Mar 30;12(3):e0174779. doi: 10.1371/journal.pone.0174779

Fig 4. Examples of delivery of diverse cargoes to CHO cells.

Fig 4

(A) Proteins of varying sizes were labelled with FITC and 4 μg protein per well was delivered to CHO cells and analyzed by fluorescence microscopy at 2 hr post-delivery, 10x magnification. (B) Increased efficiency of delivery of beta-lactoglobulin was demonstrated with increasing concentration of protein delivered. (C) Full length anti-rabbit-Alexa488 secondary antibody was successfully delivered. (D) GFP mRNA (5 μg) was delivered twice (10 μg/well in total) into cells using the permeabilization method and expression of GFP protein was observed by fluorescence microscopy at 24 hr post-delivery. (E) Luciferase mRNA (5 μg) was delivered twice (10 μg/well total) into cells using the permeabilization method and expression of luciferase was quantified by luminometry at 24 hr post-delivery. For lipofection, luciferase mRNA (5 μg) was delivered per well. (F) pGFP (5 μg) was delivered twice (10 μg/well total) into cells using the permeabilization method and expression of GFP protein was observed by fluorescence microscopy at 24 hr post-delivery. (G) pGLuc (10 μg) was delivered into cells using the permeabilization method and expression of luciferase was quantified by luminometry at 24 hr post-delivery. For lipofection, 0.5 μg pGLuc was delivered per well. n = 3, data are depicted as the mean ± standard deviation. (GFP = green fluorescent protein; pGFP = plasmid encoding GFP; pGLuc = plasmid encoding Guassia luciferase).