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. Author manuscript; available in PMC: 2018 Mar 15.
Published in final edited form as: J Immunol. 2017 Feb 10;198(6):2244–2248. doi: 10.4049/jimmunol.1502266

FIGURE 2.

FIGURE 2

Exogenous AID expression cannot restore CSR in Sox2 overexpressing cells. (A) Representative flow cytometry of splenic B cells co-infected with pMIR, pMIR-Sox2 (Sox2), pMIG, and pMIG-AID (AID). mCherry from pMIR and GFP from pMIG-based vectors served as surrogates for infection. (B) Average CSR in co-infected cells with CSR in pMIR/pMIG co-infected cells set at 100%; n=3. (C) Representative immunoblot showing expression of exogenous Sox2 and AID in the experiment from (A). (D) IgM and IgG1 germline transcripts (GLTs) were analyzed at 72h post-infection following sorting of infected cells based on mCherry expression. For qPCRs, the pMIR sample was set to 1 (n = 3).