Table 2.

Recombinant VZV glycoprotein mutants and their effect on replication in cell culture, human skin and dorsal root ganglia.

ORF [Glycoprotein]	Mutation	Phenotype			Reference
		Cell culture	Skin	DRG
ORFS/L	ΔTMD	WT in MeWo	-	-	[21]
ORF5[gK]	ΔORF5	Lethal	N/A	N/A	[17, 20]
	ΔNgK	Lethal	N/A	N/A
	ΔCgK	Lethal	N/A	N/A
	ΔCgK5251	Lethal	N/A	N/A
ORF9A[gN]	ΔgNA	Lethal	N/A	N/A	[20]
ORF14[gC]	ΔgC	WT	Reduced	N/A	[20]
	ORF14stop	WT	-	-	[64]
ORF31[gB]	ΔgB	Lethal	N/A	N/A	[20]
	W180G	Lethal	N/A	N/A	[28]
	Y185G	Lethal	N/A	N/A
	491GSGG494	WT	Reduced	-
	491ΔRSRR494	WT	Reduced	-
	Δ36 (gB-36)	Hyperfusion	-	-	[80]
	Y881F	Hyperfusion	Reduced	-	[13]
	Y920F	WT	WT	-
	Y881/920F	Hyperfusion	Reduced	-
ORF37[gH]	ΔgH	Lethal	N/A	N/A	[19, 20]
	αX	WT	Reduced	-	[19]
	S42A	WT	WT	-
	N45A	WT	WT	-
	S47A	WT	Reduced	-
	S47T	WT	WT	-
	T127A	WT	WT	-
	C327A	WT	WT	-
	T351A	WT	WT	-
	C647A	Small Plaque	Reduced	-
	S694F	-	-	-
	C703A	Small Plaque	Reduced	-
	S694F/C724A	-	-	-
	C727A	Small Plaque	Reduced	-
	S694F/C727A	WT	Reduced	-
	T751A	Small Plaque	WT	-
	α8	Lethal	N/A	N/A
	α9	Lethal	N/A	N/A
	α12	Lethal	N/A	N/A
	α14	Lethal	N/A	N/A
	C540A	Lethal	N/A	N/A
	C575A	Lethal	N/A	N/A
	C724A	Lethal	N/A	N/A
	781FPNG784	Lethal	N/A	N/A
ORF37[gH]	gH-TL (834 stop)	Hyperfusion	Reduced	-	[81]
	Δ834–841	Hyperfusion	Reduced	-
	834StopV5	Hyperfusion	-	-
	gH-V5	WT	WT	-
	Y835A	WT	WT	-
	Y835F	WT	WT	-
ORF39	ΔORF39	Lethal	N/A	N/A	[20]
ORF50[gM]	ΔgMB	Lethal	N/A	N/A	[20]
	gMim[V42P,G301M]	Small plaque	-	-	[18]
	ORF50AS(−)	WT	-	-
ORF60[gL]	ΔgL	Lethal	N/A	N/A	[20]
ORF67[gI]	ΔgI	Small Plaque	Reduced	Reduced	[16, 20, 25, 31]
	Δ37–167	Small Plaque	-	-	[29]
	Δ105–125	Small Plaque	Reduced	-
	C83A	WT	-	-
	C95A	Small Plaque	Reduced	-
	C106A	WT	WT	-
	C200A	WT	-	-
	N116A	WT	WT	-
ORF68[gE]	ΔgE	Lethal	N/A	N/A	[16, 20]
	Insertion G16	Lethal	N/A	N/A	[22]
	Insertion P27	Reduced	Reduced	-
	Insertion Y51	WT	WT	-
	Insertion G90	WT	-	-
	Insertion I146	WT	-	-
	Insertion P187	WT	-	-
	S31A	WT	Reduced	-
	S49A	WT	WT	-
	ΔP27-Y51	WT	-	-
	ΔY51-P187	Reduced	Not recovered	-
	ΔP27–P187	Lethal	N/A	N/A
	ΔCys208–236	Reduced in HELFs	Reduced	Delayed	[23, 32]
	ΔP27-G90	Small Plaque	Reduced	-	[24]
	ΔY51-G90	Small Plaque	WT	-
	Δ32–71	Small Plaque	-	-	[84]
	Δ163–208	Lethal	N/A	N/A
	Y582G (YAGL)	Lethal	N/A	N/A	[26]
	gEΔcyt	Lethal	N/A	N/A
	Y569A (AYRV)	WT	WT	WT	[26, 32]
	593AEAADA598	Increased	Increased	WT

^AAlso deletes a portion of ORF8.

WT – Wild type, not different from parental virus. N/A – Not applicable. Not tested (−)

^BAlso deletes a portion of ORF51.

WT – Wild type, not different from parental virus. N/A – Not applicable. Not tested (−)