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. 2017 Mar 20;6:e22767. doi: 10.7554/eLife.22767

Author response image 4. Titration experiments confirm the impact of HDAC inhibition on MNase protection of Igll1 promoter chromatin.

Author response image 4.

MNase titration followed by quantitative PCR showed that 1ng/ml TSA for 24 hours did not significantly affect protection of 80-120 bp amplicons (short, left) but significantly reduced protection of 130-140 bp amplicons (long, right) at the Igll1 promoter. Mean ± SE of 3 independent biological replicates.

DOI: http://dx.doi.org/10.7554/eLife.22767.028