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. 2017 Mar 20;6:e22767. doi: 10.7554/eLife.22767

Author response image 2. Endogenous versus exogenous Ikaros expression.

Author response image 2.

Western blotting for exogenous Ikaros (anti-HA), total Ikaros (Ikaros C-terminal antibody), the cytoplasmic marker Tubulin and the nuclear marker histone H3 in whole cell lysate (W), cytoplasmic (C) and nuclear (N) fractions before and after 4-OHT addition. Mobility distinguishes endogenous Ikaros from the HA-Ikaros-ERt2 fusion protein.

DOI: http://dx.doi.org/10.7554/eLife.22767.026