Video 1. Reconstructed image time lapse of single molecule localizations from live cell measurements of BCR (magenta) and PM (green).
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DOI: 10.7554/eLife.19891.022
Individual images are reconstructed using 100 frames (2 s) of single molecule images, receptors are crosslinked at time = 0, and the scale-bar is 5 µm. Single BCR or PM proteins are imaged over multiple frames and each produce clouds of localizations whose extent depend on the protein mobility as well as the typical time that a probe remains activated. This combined with under-sampling of the molecules that are present gives rise to the self-clustered appearance of probes (especially BCR) prior to receptor clustering. Overall, there is not an obvious reorganization of PM after BCR is clustered.