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. 2017 Feb 27;127(4):1316–1320. doi: 10.1172/JCI82905

Figure 2. RPS14 is required for megakaryocyte maturation.

Figure 2

(A) Fold change in expression of myeloid genes in megakaryocytes derived from PMF specimens or healthy control CD34+ cells. Results were derived from the gene-expression arrays. (B) GSEA analysis revealed that the RPS14_DN.V1_UP pathway (RPS14 gene signature) is significantly enriched. NES, normalized enrichment score; FDR, false discovery rate. (C) CD34+ cells were transduced with a control lentivirus or a lentivirus encoding either shRPS14 G5 or shRPS14 G8 and cultured under megakaryocytic differentiation conditions including 10 ng/ml THPO. Two representative experiments of 3 are shown. Megakaryocyte differentiation was reduced by 47% ± 24% in the presence of shRPS14. n = 3. P = 0.03, Student’s t test. (D) Protein synthesis assay comparing PMF megakaryocytes with controls. Top panel: flow cytometry analysis of OP-PURO fluorescence in control versus PMF 28 CD42+ megakaryocytes. Bottom panel: mean OP-Puro fluorescence intensity for controls versus PMF samples.