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. 2017 Mar 20;127(4):1425–1437. doi: 10.1172/JCI90644

Figure 3. Syngeneic IDH-MUT murine gliomas demonstrate reduced chemokine- and IFN-γ pathway–related genes and CTL infiltration.

Figure 3

C57BL/6 mice received intracranial injections of 1 × 105 GL261 or SB28 glioma cells stably transfected with cDNA for either WT or the R132H form of IDH1. Day-21 tumors were removed from mice and further assessed. (A) RT-PCR analysis of SB28 tumor–derived RNA showing decreased expression of CTL response–related genes in SB28-MUT (MUT tumor; n = 4) versus SB28-WT (WT tumor; n = 4) tumors. (B) CXCL10 ELISA was performed on tumor-derived protein extracts from mice bearing GL261-WT or GL261-MUT tumors. (C) Representative fluorescence microscopy images from sections stained with DAPI (blue), CD3 (magenta), and CD8 (yellow) on frozen tumor sections from mice bearing GL261-WT or GL261-MUT tumors. Cells were imaged as described in Figure 2. Scale bars: 10 μm. (D) Quantification of CD3+CD8+ dp cells from GL261-WT (n = 14) and GL261-MUT (n = 16) tumors using StrataQuest software. P values were obtained using a 2-sided, unpaired t test.