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. 2017 Mar 6;127(4):1475–1484. doi: 10.1172/JCI90193

Figure 2. Overexpression of ddrgk1 mRNA rescues the ddrgk1 knockdown cartilage phenotype in zebrafish.

Figure 2

(A) Ventral view at 120 h.p.f. of Alcian blue–stained WT embryos and embryos injected with either control MO or ddrgk1 MO with or without exogenous ddrgk1 mRNA. M, Meckel’s cartilage; CH, ceratohyal cartilage; N, neurocranial cartilage; CB, ceratobranchial cartilage. Scale bars: 100 μM. (B) Quantification of the craniofacial phenotype in the embryos. Embryos with normal craniofacial features had properly formed Meckel’s cartilage, ceratohyal cartilage, neurocranial cartilage, and ceratobranchial cartilage. Embryos lacking ceratobranchial cartilage showed mild craniofacial defects, while embryos with several craniofacial defects had no neurocranial or ceratobranchial cartilage and poorly developed Meckel’s cartilage and ceratohyal cartilage. WT, n = 44; 5 pg control MO, n = 36; 3 pg ddrgk1 MO, n = 50; 4 pg ddrgk1 MO, n = 47; 5 pg ddrgk1 MO, n = 43; 5 pg ddrgk1 MO + 25 pg ddrgk1 mRNA, n = 45; 5 pg ddrgk1 MO + 50 pg ddrgk1 mRNA, n = 38. *P < 0.05; ***P < 0.001; Kruskal-Wallis rank-sum test followed by Wilcoxon’s rank-sum test with continuity correction.