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. 2017 Mar 6;127(4):1271–1283. doi: 10.1172/JCI88442

Figure 6. LA1 improves vasorelaxation in mice.

Figure 6

(A) Quantification of acetylcholine-dependent (Ach-dependent) relaxation after phenylephrine (PE) precontraction in aortic rings from 19-week-old MRL/Lpr mice after 10-week treatment with LA1 or vehicle. Control data are from MRL/Mpj. Data are mean ± SEM (n = 7 LA1-treated, n = 8 vehicle-treated). (*P < 0.05, **P < 0.01, ***P < 0.001, comparing LA1 vs. controls.) Curves were first analyzed using an asymmetric (5 parameters) logistic equation; significance was determined by 2-way ANOVA. (B) Representative photomicrographs of balloon-injured arteries from rats treated with vehicle or LA1. Images of arteries stained with Evans blue vital staining in situ before sacrifice are shown at the top. Photomicrograph of cross section of arteries stained with anti-vWF antibody to label the endothelium (arrow) is presented below. Scale bars: 50 μm. (C) Quantification of the amount of Evans blue extracted from the balloon-injured arteries from rats treated with vehicle or LA1 (as in B). Data are mean ± SEM (n = 7 vehicle-treated, n = 8 LA1-treated). (D) Quantification of the extent of reendothelialization 7 days after balloon injury from the balloon-injured arteries from rats treated with vehicle or LA1 after staining with anti-vWF antibody (as in B). Data shown are mean ± SEM (n = 7 vehicle-treated, n = 8 LA1-treated) (C and D, *P < 0.05, Student’s t test).