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. 2017 Mar 6;127(4):1271–1283. doi: 10.1172/JCI88442

Figure 7. LA1 reduces IFN-I levels and the nuclear exclusion of FOXO3 in LPS-stimulated patient cells.

Figure 7

(A) Real-time qRT-PCR–based measurement of IFNB and IRF7 in PBMCs from normal donors carrying nonvariant or variant alleles of ITGAM. The data shown are expressed as fold change of ITGAM variant SNP carriers over noncarriers (normalized to 1). Data shown are mean ± SEM (major allele carriers, n = 5, and minor allele carriers, n = 6). (B) Representative immunofluorescence images of subcellular localization of FOXO3 (red, bottom panels) in human macrophages from donors carrying nonvariant (Noncarriers) or variant alleles (SNP carriers) of ITGAM treated with vehicle DMSO, LPS (50 ng/ml), or LPS (50 ng/ml) plus LA1 (20 μM) for 4 hours. Nuclei were stained with DAPI (blue). Scale bars: 10 μm. Bar graphs represent quantitation of the nuclear fraction of FOXO3 in cells (n = 30) from 3 unique donors in each group. Data are mean ± SEM (*P < 0.05, **P < 0.01, ****P < 0.0001, Student’s t test).