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. 2017 Mar 13;127(4):1505–1516. doi: 10.1172/JCI88574

Figure 2. SIRT2 mediates iron export through FPN1.

Figure 2

(A) mRNA levels of genes involved in iron homeostasis in Sirt2+/+ and Sirt2–/– MEFs (n = 5–9 for each genotype). Exoc6, exocyst complex component 6; Dmt1, divalent metal transporter 1; Ftl, ferritin light chain. (B) FPN1 protein levels in Sirt2+/+ and Sirt2–/– MEFs (n = 3 per group). (C) Representative blot from 3 independent experiments showing FPN1 levels in HepG2 cells treated with lenti-control shRNA or lenti-SIRT2 shRNA. (D) mRNA levels of FPN1 in HepG2 cells infected with lenti-control shRNA or lenti-SIRT2 shRNA (n = 5 per group). (E) Time-dependent 55Fe export in HepG2 cells treated with lenti-control shRNA or lenti-SIRT2 shRNA after 16 hours of incubation with radioactive iron (n = 6 per group). Data are presented as the mean ± SEM. *P < 0.05, by Student’s t test.