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. 2017 Mar 13;127(4):1505–1516. doi: 10.1172/JCI88574

Figure 3. Regulation of Fpn1 by SIRT2 occurs through NRF2.

Figure 3

(A) Fpn1 mRNA levels in MEFs infected with lenti-control shRNA or lenti-Nrf2 shRNA (n = 5–6 per group). (B) Representative blot from 2 independent experiments showing FPN1 expression in Sirt2+/+ and Sirt2–/– MEFs infected with lenti-control shRNA or lenti-Nrf2 shRNA. (C) NRF2 protein levels in Sirt2+/+ and Sirt2–/– MEFs (n = 3 per genotype). (D) mRNA levels of Nrf2 in Sirt2+/+ and Sirt2–/– MEFs (n = 8 per genotype). (E) NRF2 protein levels in nuclei of Sirt2+/+ and Sirt2–/– MEFs (n = 3 per genotype). (F) NRF2 antioxidant target genes Nqo1 and Hmox1 mRNA levels in Sirt2+/+ and Sirt2–/– MEFs (n = 8–9 per genotype). (G) Non-heme iron levels in Sirt2+/+ and Sirt2–/– MEFs infected with lenti-control shRNA or lenti-Nrf2 shRNA (n = 6 per genotype). (H) Luciferase activity in HEK293T cells transfected with a murine Fpn1 promoter–luciferase reporter construct along with Nrf2 plasmid and WT Sirt2 or deacetylation-null Sirt2 plasmids (lenti-Sirt2-WT and lenti-Sirt2-DN). An empty vector was used as a control (n = 4–6 per group). Data are presented as the mean ± SEM. *P < 0.05, by ANOVA with Bonferroni’s correction for multiple comparisons (A, G, and H) or by Student’s t test (C, D, and F).