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. 2017 Mar 13;127(4):1353–1369. doi: 10.1172/JCI85963

Figure 1. HDAC6 is a negative regulator of lipid storage.

Figure 1

(A) Levels of HDAC6 and HDAC10 protein were decreased in the GWAT of leptin-deficient (ob/ob) mice (n = 3 mice per group). (B) Reduced expression of HDAC6 and HDAC10 in the visceral fat of obese rhesus monkeys and the intra-abdominal fat of humans (n = 3 samples per group). (C) Levels of HDAC6 protein in the GWAT and the BAT of control (Ctrl) and Hdac6 AKO mice (n = 3 mice per group). Ac-αTub, acetlyated α-tubulin. (D) Representative photograph of 4-month-old control and Hdac6 AKO mice. (E) Growth curve (n = 6 mice per group) reflected by body weight analysis (n = 10 mice per group) for control and the Hdac6 AKO mice fed ND or HFD. Two-way repeated-measures ANOVA was used to evaluate the data (left panel); #P < 0.05, ##P < 0.01. For the for right panel, 2-tailed Student t test was used; *P < 0.05, **P < 0.01, ***P < 0.001. (F) Adiposity index for control and Hdac6 AKO mice (n = 6 mice per group). (G) Fat mass and lean mass by MRI scanning of control and Hdac6 AKO mice (n = 6 mice per group). (H) Morphology of the GWAT and BAT of control and Hdac6 AKO mice (n = 3 mice per group). Scale bars: 50 μm. (I) Total TAG levels of GWAT, BAT, liver, and muscle of control and Hdac6 AKO mice (n = 6 mice per group). Data represent the mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001, by 2-tailed Student’s t test. One-way ANOVA with Dunnett’s correction was used for multiple comparisons (F and G).