Skip to main content
. 2017 Mar 20;127(4):1574–1588. doi: 10.1172/JCI89535

Figure 7. DLL4 expression within different LN nonhematopoietic cell populations after allogeneic BMT.

Figure 7

(A) LN stromal cell subsets. TgCcl19-Cre+ Dll1Δ/Δ Dll4Δ/Δ and TgCcl19-Cre– littermate control mice were lethally irradiated before infusion of allogeneic BALB/c splenocytes. LNs were collected on day 1.5 after transplantation and enzymatically digested. Dot plots show representative flow cytometric analysis of CD45–Ter119– nonhematopoietic stromal cells. (B) DLL4 expression in LN-resident nonhematopoietic cell subsets. Roman numerals refer to the cell populations shown in A. (C) MFI comparing DLL4 expression in stromal cell subsets from TgCcl19-Cre– versus TgCcl19-Cre+ Dll1Δ/Δ Dll4Δ/Δ mice as well as isotype staining in controls. **P < 0.01 and ***P < 0.001, by unpaired 2-tailed Student’s t test with Sidak’s correction for multiple comparisons. (D) Representative flow cytometric plots of PDPN+CD31–CD21/35– FRCs showing DLL4 expression in the CD157hi subset. Data are representative of at least 3 experiments.