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. Author manuscript; available in PMC: 2017 Mar 30.
Published in final edited form as: Biochim Biophys Acta. 2016 Mar 31;1861(9 Pt B):1352–1364. doi: 10.1016/j.bbalip.2016.03.027

Figure 2. Sec14-homology proteins of rice, birdsfoot trefoil, tobacco, and soybean.

Figure 2

Database searches were as described in the legend to Figure 1, and Figure labeling is also as described in Figure 1. The asterisk in the LjPLP-I denotes an amber stop in the Sec14-domain coding sequence (55), and the Lotus japonicus genome assembly build 3.0 tool (http://www.kazusa.or.jp/lotus/blast.html) predicts two annotated genes (Lj4g3v0988870.1 and Lj4g3v0988870.2, rather than a single Sec14-nodulin gene as shown here. The .1 and .2 suffixes indicate alternative splicing from the same pre-mRNA. The predicted Lj4g3v0988870.1 translation product is a 384 polypeptide containing the N-terminal 159 residues of the Sec14 domain, while the Lj4g3v0988870.2 is predicted to encode a 314 amino acid polypeptide consisting of a 79 residue C-terminus from the Sec14-domain joined to the nodulin-domain. As translational read-through of translation stop codons is known, we list the full-length LjPLP-I polypeptide (550 amino acids) as such read-through could potentially generate such a translation product at some low frequency. Thus, the Sec14-nodulin protein family of Lotus consists of at least three, and perhaps four, proteins. Abbreviations: Os, Oryza sativa; Lj, Lotus japonicus; Nb, Nicotiana benthamiana; Ssh, soybean Sec14 homolog.