Fig 2.

Sleep state power spectra (0.5-20 Hz frequency range) during dark and light periods of baseline spontaneous sleep in WT and NLRP3 KO mice. WT and NLRP3 KO mice demonstrated significantly greater EEG power spectra during NREM sleep and wake dark periods compared to the light periods in both genotypes (A, B, E, F), although little difference was found in REM sleep EEG power spectra (C, D) between light and dark periods for either genotype. However, NLRP3 KO mice exhibited some diurnal differences in particular power spectra bands during these sleep states compared to KO mice [light period vs. dark period: F (1, 553) = 52.007, p < 0.001; light period vs. dark period × frequency interaction: F (78, 553) = 4.750, p < 0.001]. Post-hoc analysis indicated that WT mice had significantly greater NREM sleep EEG power spectra in the 1.25 Hz frequency bands during the dark period vs. the light period. NLRP3 KO mice also exhibited increases in NREM sleep EEG power spectra. Post-hoc analysis also revealed that EEG power spectra during wakefulness was greater in the 8.25-8.75 Hz frequency bands during the dark period vs. light period in WT mice. N = 8 per genotype. Bars and (*) indicates significant difference between genotypes for individual EEG power spectra. The Bonferroni corrected α value was set at p < 0.0006.