Table 10.

Library matched metabolites that are deemed to be important for separating pregnant rats administered a single p.o. (10 mg/kg) dose of 20 nm AgNP from vehicle at 48 h. Metabolites often fall in multiple bins, which is noted by the parenthesis.

	AgAc vs Vehicle		20 nm AgNP vs Vehicle		110 nm AgNP vs Vehicle
Library Matched Metabolite	VIPa Range	Fold Change rangeb	VIP Range	Fold Change range	VIP Range	Fold Change range
1-Methylnicotinamide | Lactose | Trigonelline	1.2	(−1.2)			1.5	(−1.1)
2-Oxoglutarate (2 bins)			3.1 – 3.3	1.5 – 1.6
3-Hydroxyphenylacetate					1.0	(−1.5)
3-Hydroxyphenylacetate | N-Acetylglutamine			1.5	(−1.6)	1.3	(−1.2)
Acetate	5.5	2.3	1.3	(−1.3)
Acetoacetate | trans-Aconitate			2.3	(−1.2)
Alanine	1.1	1.1			1.1	1.0
Allantoin	3.3	(−1.7)			1.3	(−1.6)
Benzorate (2-3 bins)	1.7 – 2.5	2.9 – 11.5			1.2 – 1.8	1.3 – 2.7
Citrate (2 bins)			4.9 – 5.6	(−1.4)
Dimethylsulfone			1.0	1.1	1.7	1.1
Glucose	1.6	(−1.4)
Glucose | Lactose			1.1	1.2
Glucose | Sucrose	2.7	1.8	1.4	1.2
Glucose | Taurine	2.2	(−1.5)
Hippurate (3 bins)					2.9 – 4.4	1.6
Hippurate | Glycolate					4.4	1.2
Homocystine					1.7	(−1.2)
Isoleucine	1.0	1.1
Lactate (2 bins)	1.8 – 3.5	1.2 −2.0	1.1	1.1
Lactose | Glucose	2.3	1.9
Leucine | Isoleucine | Fatty acids			1.7	1.3	2.0	1.2
N,N-Dimethylglycine	2.1	1.4
N-Acetylglutamine	1.5	(−1.1)	1.5	(−1.2)	1.8	(−1.1)
N-Acetylglycine | N-Acetylaminoacids			1.2	(−1.0)
Proline			1.0	(−1.1)	1.3	1.1
Succinate	4.9	2.0	2.8	(−1.6)
Sucrose			1.5	1.3
Sucrose | Creatinine			1.6	1.2
Trans-Aconitate			1.2	(−1.4)
Valine/Leucine			1.4	1.4
Unknowns (3-8 bins)	1.0 – 2.8	(−1.6) – 1.5	1.1 – 3.4	(−1.1) – 1.2	1.4 – 2.1	(−1.2) – 1.2

^aVIP = variable importance for projection

^bA negative fold change means that the vehicle median was higher than the AgNP median, while a positive fold change means that the AgNP median was higher than the vehicle median.