Figure 4.

Anti-Ad5 serum neutralizing antibody titers in responder-subjects. Anti-Ad5 neutralizing serum antibodies employed a previously described assay ^36,37. The assay tests the ability of serum dilutions to block the transduction of 293 cells by an Ad5 vector, AdCMVLuc encoding luciferase. The titers (Y-axis) represent serum dilutions resulting in a 50% inhibition of transduction. The X-axis represents time points in the study. B = baseline, while all other time points shown are days following AdhAQP1 administration. Individual subject symbols are indicated. No sample was available to perform this assay at the second follow-up visit of subject 19. The exact days of each follow-up visit are listed in the legends of Figures 1 and 3, and Table 1.Note that serum neutralizing antibody titers found in all time points through day 42 have recently been reported and were generally similar to those shown in the figure ¹⁶.