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. Author manuscript; available in PMC: 2017 Jun 12.
Published in final edited form as: Nat Mater. 2016 Dec 12;16(4):419–425. doi: 10.1038/nmat4829

Figure 3. Squamous cyst development is transcriptionally distinct from canonical EMT or primitive streak.

Figure 3

(a) Confocal micrographs showing immunostaining of BRACHYURY (BRA, red; top), SNAIL (red; middle), and SLUG (red; bottom) in hPSCs cultured under indicated conditions and corresponding staining of PS cells derived from hPSCs under 2D culture16 (PS-2D). HOECHST (blue) counterstains nuclei. (b) Confocal micrographs showing immunostaining of ECAD (green; top) and N-CADHERIN (NCAD, red; bottom) for hPSCs under indicated culture conditions. HOECHST (blue) counterstains the nuclei. Scale bars in a and b, 50 μm. n = 2 independent experiments. (c&d) Schemes summarizing the transcriptional control program for canonical EMT (c) and the columnar-to-squamous transition reminiscent of human amnion development (d).