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. Author manuscript; available in PMC: 2017 Sep 6.
Published in final edited form as: Nat Genet. 2017 Mar 6;49(4):635–642. doi: 10.1038/ng.3805

Figure 4.

Figure 4

Quantitative estimation of cancer DNA proportion in cell-free DNA based on MHL of informative MHBs. (a) Colorectal cancer (b) Lung cancer. Informative MHBs were selected based on the presence of high-MHL in cancer solid tissues (CT) and the absence of MHL in whole blood (WB). Group II regions have high MHL in cancer tissues (MHL>0.5) and cancer plasma while low MHL in WB and normal tissues (MHL<0.1), and hence were selected for further analysis. Bar-plots show average MHL in different groups of samples. MHL in cancer plasma (CCP and LCP) and normal plasma (NP) were compared with a two-tail t-test. NCT denotes normal colon tissues, NLT denotes normal lung tissues, and ONT denotes other normal tissues. (c) MHL has higher signal-to-noise ratio (Mean/SD) than individual 5mC levels as tumor fraction decreases. X-axis is the tumor fraction in synthetic mixtures. (d) Estimation of the cancer DNA proportions in plasma samples. CCP denotes colorectal cancer plasma, LCP denotes lung cancer plasma, and NP denotes normal plasma.