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. Author manuscript; available in PMC: 2017 Apr 17.
Published in final edited form as: Oncogene. 2016 Oct 17;36(13):1760–1769. doi: 10.1038/onc.2016.350

Figure 3. MDM2 maintains retinoblastoma cell proliferation in part by promoting MYCN expression.

Figure 3

A. Western analysis at day 4 (left) and cell growth response (right) of RB176 cells after infection with lentivirus expressing shRNA against MYCN or a scrambled control (Scr). B. Tumors formed by RB176-luc cells transduced with shScr but not by those transduced with shMYCN in mouse subretinal xenografts (right, P-value from two-tailed Fisher’s exact test). Western analysis of the cells used for xenograft (left). C. Western analysis at day 4 (left) and cell growth response (right) of CHLA-RB215 cells after infection with lentivirus expressing shRNA against MYCN or Scr. D. Western analysis at days 4 and 25 (left and middle) and cell growth response (right) of RB176 cells after co-infection with lentivirus expressing shRNA against MDM2 or Scr and with lentivirus expressing MYCN under control of the EF1α promoter (BN-MYCN) or the empty vector (BN). E. Western analysis at day 4 (left) and cell growth response (right) of RB176 cells after co-infection with lentivirus expressing shMDM2 or Scr and with lentivirus expressing shp27 or Scr.