Figure 3. The histological defects in Emx1-Cre Cic mutant animals occur postnatally in post-mitotic neurons.

(a) The numbers of CUX1⁺ cells and SATB2⁺ cell in layer 2–4 diminished postnatally. Immunofluorescent staining with CUX1 and SATB2 revealed that the number of CUX1⁺ cells and SATB2⁺ cells at P0 were comparable among control and mutant animals. The number gradually decreased in the mutant mice, and by P10 the numbers were comparable to those from 5-week old animals (n = 4–6, scale bar = 100 μm). (The 5-week old data are from Figure 2.) (b) Immunofluorescent staining with CUX1 and SATB2 revealed that the numbers of layer 2–4 CUX1⁺ and SATB2⁺ cells were reduced in Neurod6-Cre conditional mutants, which removes Cic in post-mitotic neurons (n = 4–5, scale bar = 100 μm). Data are represented as mean ± s.e.m. *: P < 0.05; ****: P < 0.0001