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. Author manuscript; available in PMC: 2017 Oct 28.
Published in final edited form as: J Tissue Eng Regen Med. 2016 Jun 7;11(10):2737–2751. doi: 10.1002/term.2168

Figure 9.

Figure 9

Neurons extend neurites directly along the stretched astrocytic processes. (A, G) Phase-contrast image and (B, H) confocal reconstruction of two separate areas of astrocytic processes that were stretched to unprecedented lengths of 1.5 mm and directed and supported neuronal survival; scale bars =200 μm. (C–F) Enlargement of boxed region in (B), showing (C) Hoechst counterstain, (D) GFAP-positive stretched astrocytic processes, (E) β-tubulin III-positive neurons and (F) overlay; scale bars = 25 μm. (H) Second area demonstrating stretched astrocytic bundles seeded with neurons were (I) stained for Hoechst, (J) labelled for GFAP, (K) β-tubulin III (Tuj1) and (L) overlay