Figure 3. Detection of a list of putatively identified positively and negatively charged lipids by LipidFinder and three commonly used processing packages.

(A) Table of reference lipids identified by lipid species and category and whether they were detected (green)/undetected (red) by each of the four programs. A series of platelet lipids was manually verified to be present in the Orbitrap dataset, using Xcalibur, and then interrogated for detection using each of the programs, as shown. (B) Bar chart summary of A results. Data shows the % of lipids in positive/negative ion mode detected by each program, calculated from A.