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. 2017 Apr 6;2(7):e91634. doi: 10.1172/jci.insight.91634

Figure 6. MS identification of eicosanoid-phosphoinositide lipids generated by platelets.

Figure 6

(A) Orbitrap MS of HETE-PIs in platelet lipid extracts at 60,000 resolution (at m/z 400) showing elution of ions with m/z values corresponding to 18:0, 18:1, and 16:0/12-HETE-PIs. (B) MS of the putative HETE-PI ions. Single ions are shown at expected m/z values, circled, for the corresponding lipids in A. (C) MS/MS of the two most abundant HETE-PIs. Ions from 12-HETE (m/z 179, 319), PI headgroup (153,241, 315), and sn1 fatty acids (283 and 281 for 18:0 and 18:1, respectively) are seen. (D and E) LC/MS/MS separation of thrombin-activated (D) or basal (E) platelet lipid extracts monitoring MRM transitions corresponding to HETE-PEs. HETE-PIs are only detected following activation of platelets.