Fig. 3.

FtMt immunohistochemistry and pre-absorption test in monkey tissues. A: Immunostaining of FtMt in the substantia nigra pars compacta of the monkey, using the C65-2 monoclonal anti-FtMt antibody. B: A high magnification of the boxed area B in Fig. 3A. FtMt-positive granules are seen in neuronal cell bodies. C and D: Immunoabsorption tests. The staining intensity of FtMt-immunoreactivity was much decreased with the use of the antibody pre-incubated with 0.4 μg/ml of the FtMt peptide (C). No positive staining was detected when the antibody was pre-absorbed with 1 μg/ml of the peptide (D). Bars = 50 μm.