Figure 4.

The Conserved ICAM-1 Binding Site Is Recognized by Patient Plasma and Linked to CM

(A) The inhibition of DBLβ domains binding to ICAM-1 by IgG from a plasma pool from Liberian P. falciparum-exposed adults purified on ICAM-1-binding DBLβ_D4 domain from Dd2var32, KM364031, and PFD1235w or on a closely related, but non-ICAM-1-binding DBLβ_D5 domain from PFD1235w. ICAM-1 inhibitory capacity is >74% (black), 51%–74% (dark gray), 21%–50% (light gray), and 0%–20% (white).

(B) ICAM-1-binding inhibition by plasma with low (ELISA OD < 1) and high (ELISA OD > 1) anti-motif-DBLβ IgG in P. falciparum-exposed Tanzanian children (1–17 years) (Lusingu et al., 2004). Boxplot with median. Whiskers, 5% and 95% percentiles.

(C) Transcript levels (in arbitrary transcription units, Tu) of var gene subtypes in Ghanaian, Tanzanian, and Beninese children hospitalized with malaria, shown with medians and 25% and 75% percentiles.

See also Table S5.