Figure 5.

PfEMP1 Can Simultaneously Bind to Both ICAM-1 and EPCR

ICAM-1^D1D5 was immobilized at fixed concentration, followed by injection of the respective head structure, (A) PF11_0521 DBLα1.7-CIDRα1.4-DBLβ3, (B) Dd2var32 DBLα1.7-CIDRα1.4-DBLβ3, and (C) PFD1235w CIDRα1.6-DBLβ3, also at fixed concentration. A 2-fold dilution series of EPCR was injected over the resulting ICAM-1::head structure complex. Arrows show start points of protein injection. The insets show data fitted to a one-site kinetic model for binding of EPCR to the respective ICAM-1::head structure complex. Data (black lines) are modeled to a one-site model (red lines). See also Figure S6 and Table S6.