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. 2016 Dec 7;595(7):2271–2284. doi: 10.1113/JP272942

Figure 1. Both FLNA and α‐actinin2 cytoskeletal proteins increase SK2 channel membrane expression in HEK293 cells.

Figure 1

A, a schematic diagram of the human SK2 channel fused with tdTomato used in the study. B, epifluorescence and TIRF images of SK2 channels expressed alone or co‐expressed with FLNA or α‐actinin2; scale bar is 20 μm. C, summary data for averaged mean fluorescence intensity from TIRF images, when SK2 channels were expressed alone or co‐expressed with FLNA or with α‐actinin2. * P < 0.05 (n = 13 cells). D, immunofluorescence confocal microscopic imaging of HEK 293 cells transfected with human cardiac SK2‐HA alone or co‐transfected with SK2‐HA and FLNA or α‐actinin2. Cells were immunostained using anti‐HA antibody followed by chicken anti‐mouse Alexa Fluor 555 secondary antibody with no permeabilization (NP). Cells were then permeabilized (P) and immunostained using anti‐HA antibody followed by rabbit anti‐mouse Alexa Fluor 633 secondary antibody. E, summary data of the fluorescence ratios (555/633) from the three groups of cells (* P < 0.05, n = 15–18 cells).