A and C, epifluorescence and TIRF images of HEK 293 cells expressing SK2 channels and FLNA (A) or α‐actinin2 (C), pretreated with vehicle, primaquine or dynasore. B and D, summary data for averaged mean fluorescence intensity from A and C, *
P < 0.05 (n = 10–12 cells). Scale bar is 20 μm. E, a schematic diagram of SK2 channels recycling and endocytosis from plasma membrane to recycling or early endosomes and the known actions of primaquine and dynasore. CaMBD in the diagram refers to calmodulin binding domain within the C terminus of SK2 channels.