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. 2016 Dec 7;595(7):2271–2284. doi: 10.1113/JP272942

Figure 3. Effects of CA and DN forms of Rab4, 5, 8, and 11 on the membrane expression of SK2 channels.

Figure 3

A, TIRF images of SK2 channels in HEK 293 cells when expressed alone or co‐expressed with FLNA or α‐actinin2. Different forms of Rab proteins including CA or DN forms of Rab4, 5, 8, and 11 were co‐expressed. B, corresponding summary data for averaged mean fluorescence intensity under TIRF imaging are presented. Statistical comparisons were made between CA and DN forms. C, SK2 channels were co‐expressed with FLNA and α‐actinin2, and with the CA or DN forms of Rab4 or Rab11 proteins. TIRF images and averaged mean fluorescence intensity are shown. Scale bar is 20 μm, * P < 0.05 (n = 14 cells for each group). D, immunofluorescence confocal microscopic imaging of HEK 293 cells transfected with human cardiac SK2‐HA or co‐transfected with SK2‐HA and α‐actinin2 or FLNA as shown in Fig. 1 D. Cells were further co‐transfected CA or DN forms of Rab11. Cells were immunostained as described in Fig. 1 D. E, summary data of the fluorescence ratios (555/633) from the three groups of cells (* P < 0.05, n = 15–18 cells per group). F, a schematic diagram of the roles of different Rab proteins in the recycling and endocytosis of ion channel proteins.