A, Kir2.1 mRNA expression in endothelial cells isolated from WT and Kir2.1+/− mesenteric arteries: a, amplification plots of qPCR; b, relative mRNA expression normalized to GAPDH (n = 3, *
P < 0.05). B, Kir2.1, 2.2 and 2.3 mRNA expression levels in endothelial cells from WT and Kir2.1+/− mice (n = 3 per group, *
P < 0.05). C, Kir2.1 protein expression in the same cells: a, representative Western blot of Kir2.1 (53 kD); b, densitometry analysis of Kir2.1 expression normalized to GAPDH (n = 3, *
P < 0.05).