Figure 6.

Suppressive effects of various pharmacological inhibitors on TGF-β1-induced increases in expression of α-smooth muscle actin (α-SMA) and type 1α1 collagen (COL1A1) in human lung fibroblasts. The inhibitors (Inhib.) used were JNK inhibitor (A; SP600125, 10 μM), p38 inhibitor (B; SB203580, 10 μM), Smad3 inhibitor (C; SIS3, 3 μM), and ERK inhibitor (D; PD98059, 10 μM). Group data of expression of α-SMA and COL1A1 are shown in (E,F). Protein samples were harvested 24 h after treatments and data were measured by Western blot analysis. Data in each group are mean ± SEM from 4 independent experiments. Note that only PD98059 had no significant effect (p > 0.05) on TGF-β1-induced increase in expression of α-SMA and COL1A1.