Table 2.
Assay | OD600 |
Resveratrol volumetric yield (μg/mL) |
Resveratrol productivity (mg/gh−1) |
Cellular viability |
Plasmid copy number (PCN) |
|||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
PI+(%) |
BOX+(%) |
pAC-4CL1 |
pUC-STS |
|||||||||||
22 h | 30 h | 22 h | 30 h | 22 h | 30 h | 22 h | 30 h | 22 h | 30h | 22 h | 30 h | 22 h | 30 h | |
1 | 1.84 | 2.53 | 57.94 ± 2.44 | 69.17 ± 1.34 | 5.73 ± 0.16 | 3.65 ± 0.31 | 1.48 | 1.43 | 23.31 | 29.7 | 26 ± 10.60 | 28 ± 0.96 | 25 ± 4.09 | 30 ± 10.15 |
2 | 2.77 | 4.43 | 84.1 ± 9.49 | 153.73 ± 1.08 | 5.52 ± 1.27 | 4.63 ± 1.90 | 5.56 | 7 | 21.32 | 15.02 | 115 ± 47.14 | 130 ± 14.93 | 20 ± 2.18 | 26 ± 5.44 |
3 | 2.72 | 4.19 | 86.3 ± 6.41 | 159.96 ± 2.86 | 5.77 ± 0.48 | 5.09 ± 0.96 | 4.3 | 7.06 | 21.66 | 18.08 | 215 ± 84.94 | 242 ± 36.44 | 72 ± 16.10 | 89 ± 15.53 |
4 | 2.24 | 2.73 | 23.51 ± 0.31 | 46.07 ± 0.65 | 1.91 ± 0.52 | 2.25 ± 0.27 | 0.28 | 1.48 | 30.23 | 27.68 | 47 ± 15.48 | 77 ± 9.61 | 95 ± 46.53 | 86 ± 11.48 |
5 | 2.35 | 2.9 | 39.38 ± 3.02 | 64.72 ± 2.24 | 3.05 ± 0.02 | 2.98 ± 0.03 | 1.18 | 3.78 | 18.84 | 20.38 | 29 ± 7.25 | 77 ± 36.28 | 22 ± 11.15 | 35 ± 9.34 |
6 | 2.76 | 4.44 | 39.48 ± 1.47 | 65.54 ± 0.63 | 2.60 ± 0.44 | 1.97 ± 0.88 | 1.88 | 3.1 | 18.58 | 20.5 | 28 ± 23.60 | 40 ± 15.39 | 4 ± 0.71 | 4 ± 1.25 |
7 | 2.76 | 4.42 | 41.71 ± 2.89 | 97.56 ± 5.61 | 2.75 ± 0.06 | 2.94 ± 0.08 | 2.08 | 3.7 | 16.08 | 14.08 | 30 ± 12.76 | 47 ± 21.25 | 23 ± 3.87 | 14 ± 6.76 |
8 | 2.13 | 3.35 | 40 ± 1.08 | 79.32 ± 3.62 | 3.41 ± 0.37 | 3.16 ± 0.55 | 3.12 | 3.7 | 9.9 | 13.94 | 51 ± 14.66 | 62 ± 5.71 | 15 ± 6.66 | 14 ± 2.27 |
9 | 2.56 | 2.86 | 28.57 ± 0.28 | 50.68 ± 1.88 | 2.03 ± 0.32 | 2.36 ± 0.09 | 1.17 | 1.26 | 30.95 | 26.7 | 33 ± 7.63 | 36 ± 17.88 | 45 ± 6.66 | 62 ± 2.27 |
10 | 2.04 | 3.16 | 28.66 ± 5.05 | 32.53 ± 3.64 | 2.55 ± 0.33 | 1.37 ± 0.16 | 5.62 | 5.1 | 19.86 | 16.14 | 218 ± 54.82 | 394 ± 74.85 | 91 ± 9.32 | 64 ± 11.60 |
11 | 4.5 | 4.9 | 1.53 ± 0.27 | 3.11 ± 2.32 | 0.06 ± 0.01 | 0.08 ± 0.01 | 3.12 | 2.4 | 21.3 | 27.34 | 117 ± 73.19 | 53 ± 16.91 | 31 ± 22.22 | 62 ± 30.71 |
12 | 2.68 | 3.02 | 56.45 ± 3.06 | 69.87 ± 5.27 | 3.83 ± 0.97 | 3.08 ± 0.50 | 1.37 | 2.13 | 23.42 | 27.08 | 51 ± 24.16 | 82 ± 11.03 | 128 ± 41.38 | 206 ± 57.15 |
13 | 2.78 | 3.9 | 74.34 ± 1.92 | 100.59 ± 7.83 | 4.86 ± 0.75 | 3.44 ± 1.35 | 2.3 | 2.42 | 24.42 | 18.8 | 34 ± 6.40 | 44 ± 7.86 | 78 ± 21.13 | 160 ± 73.50 |
14 | 2.54 | 3.41 | 23.71 ± 4.15 | 78.01 ± 5.08 | 1.70 ± 0.43 | 3.05 ± 0.47 | 1.24 | 2.83 | 27.63 | 33.04 | 64 ± 4.93 | 81 ± 4.69 | 177±35.29 | 239 ± 34.75 |
15 | 2.24 | 2.31 | 52.24 ± 9.92 | 109.28 ± 2.44 | 4.24 ± 0.15 | 6.31 ± 0.69 | 1.87 | 2.94 | 24.9 | 31.11 | 48 ± 10.12 | 67 ± 13.21 | 103 ± 53.40 | 135 ± 81.07 |
16 | 1.42 | 2.82 | 22.21 ± 1.72 | 26.32 ± 0.86 | 2.84 ± 0.28 | 1.24 ± 0.85 | 3.76 | 6.13 | 19.82 | 30.87 | 81 ± 6.62 | 113 ± 9.87 | 44 ± 14.56 | 69 ± 7.81 |
17 | 4.65 | 4.64 | 32.37 ± 1.92 | 35.06 ± 2.80 | 1.27 ± 0.03 | 1.01 ± 0.16 | 3.9 | 5.9 | 22.8 | 43.2 | 64 ± 2.01 | 94 ± 18.35 | 69 ± 78.15 | 59 ± 24.94 |
18 | 4.04 | 3.97 | 58.52 ± 0.53 | 61.99 ± 0.99 | 2.63 ± 0.03 | 2.08 ± 0.42 | 4.58 | 8.28 | 23.98 | 32.9 | 55 ± 30.94 | 96 ± 8.80 | 28 ± 11.43 | 33 ± 7.82 |
19 | 1.82 | 3.1 | 9.32 ± 0.75 | 13.41 ± 0.97 | 0.93 ± 0.21 | 0.58 ± 0.46 | 3.14 | 4.32 | 10.02 | 16.78 | 84 ± 27.46 | 44 ± 5.94 | 40 ± 4.00 | 21 ± 4.95 |
20 | 3.09 | 3.59 | 17.51 ± 1.22 | 15.90 ± 0.17 | 1.03 ± 0.12 | 0.59 ± 0.19 | 1.94 | 4.07 | 21.19 | 39.07 | 48 ± 16.73 | 36 ± 2.86 | 10 ± 8.92 | 5 ± 0.93 |
21 | 3.04 | 3.87 | 13.81 ± 1.72 | 23.22 ± 1.96 | 0.83 ± 0.33 | 0.80 ± 0.85 | 2.93 | 5.88 | 8.82 | 18.8 | 17 ± 3.60 | 14 ± 1.10 | 48 ± 12.05 | 39 ± 20.97 |
22 | 3.85 | 3.66 | 21.17 ± 1.69 | 20.18 ± 1.15 | 1.00 ± 0.11 | 0.74 ± 0.08 | 2.78 | 4.48 | 18.34 | 13.32 | 53 ± 16.21 | 31 ± 8.37 | 33 ± 6.93 | 14 ± 5.75 |
23 | 3.15 | 4.76 | 11.69 ± 0.52 | 14.90 ± 2.23 | 0.67 ± 0.21 | 0.42 ± 0.39 | 5.1 | 3.5 | 38.58 | 50.16 | 75 ± 34.75 | 107 ± 42.68 | 71 ± 13.66 | 88 ± 19.65 |
24 | 3.74 | 3.84 | 16.13 ± 1.27 | 15.77 ± 2.82 | 0.78 ± 0.07 | 0.55 ± 0.24 | 4.3 | 5.72 | 27.4 | 21.76 | 42 ± 5.80 | 55 ± 5.07 | 32 ± 4.50 | 25 ± 4.56 |
25 | 3.84 | 4.07 | 13.59 ± 2.63 | 31.72 ± 3.47 | 0.64 ± 0.25 | 1.04 ± 0.03 | 2.42 | 4.6 | 11.51 | 21.78 | 3 ± 0.43 | 2 ± 0.10 | 14 ± 2.86 | 40 ± 31.24 |