Skip to main content
. 2016 Dec 15;312(3):L309–L325. doi: 10.1152/ajplung.00357.2016

Fig. 2.

Fig. 2.

Effects of capsaicin on single-channel activity in membrane patches of human PASMCs. A and D: representative current records for normal PASMCs and IPAH PASMCs in a cell-attached membrane patch at various test potentials ranging from +20 to +100 mV in 20-mV steps (A and D, left). Composite current-voltage relationship (I-V curve) indicates the slope conductance of this capsaicin-sensitive channel is 143 pS for normal PASMCs and 144 pS for IPAH PASMCs (A and D, right). B and E: representative unitary currents in a cell-attached membrane patch elicited by a sustained depolarization at +80 mV for normal PASMCs (B) and IPAH PASMCs (E) exposed to vehicle (control), 1 µM capsaicin (CPS), or 1 µM capsaicin plus 10 µM capsazepine (CPS+CPZ). C and F: bar graphs showing the steady-state open probability [P(o)] of normal PASMCs (C) and IPAH PASMCs (F) as a function of time for the sample recordings in B and E, respectively. n = 4–6 cells per group.