Fig. 4.

Inhibition of HDAC6 activity decreases renal tubular cell apoptosis in GL-induced AKI. A: photomicrographs illustrate TdT-mediated dUTP nick-end labeling (TUNEL) staining of kidney tissue collected at 48 h after sham and GL administration with/without TA treatment. B: the kidney tissue lysates were subjected to immunoblot analysis with specific antibodies against cleaved caspase-3 and β-actin. C: positive TUNEL staining cells were counted and expressed as means ± SE. D: expression level of cleaved caspase-3 was quantified by densitometry and normalized with β-actin. Data are represented as the means ± SE (n = 6). Means with different superscript letters are significantly different from one another (P < 0.05).