Fig. 11.

MG132 significantly upregulated expression of HDAC6 in human tubular epithelial cell. HK2 cells were exposed to cisplatin and TA in the absence or presence of MG132 (5 μM) for 24 h. A: cell lysates were subjected to immunoblot analysis with specific antibodies against HDAC6 and β-actin. B: expression level of HDAC6 was quantified by densitometry and normalized with β-actin. Data are represented as the means ± SE (n = 4). Means with different superscript letters are significantly different from one another (P < 0.05). The results indicated that TA treatment reduces HDAC6 expression and in the presence of MG132 HDAC6 expression levels are partially resumed.