Fig. 6.
Canonical Wnt/β-catenin pathway inhibitor DKK1 blocked mRNA and protein expression of EndMT-related markers after PTH treatment in aortic ECs. A–C: real-time PCR analyses showed mRNA expression of CD31 (A), FSP1 (B), and α-SMA (C) after various treatments for 48 h with 10−7 mol/l PTH with or without 500 ng/ml DKK1 as indicated. D and E: Western blot analyses showed protein expression of CD31, FSP1, and α-SMA in different groups as indicated. Representative Western blots (D) and quantitative determinations of protein levels (E) are presented. The data are expressed as the means ± SDs (n = 3). *P < 0.05 vs. CTL group; #P < 0.05 vs. PTH group.