Fig. 3.

Deletion of Cav1 from mouse intestinal epithelial cells (CAV1^IEC-KO). (A) Schematic representation of deletion of Cav1 in the intestinal epithelium. (B) PCR of genomic DNA from whole mouse jejunum shows that Cre recombination of Cav1 has occurred in CAV1^IEC-KO jejunum but not in Cav1^fl/fl WT littermates. (C) Cav1 mRNA is decreased 68% in CAV1^IEC-KO mouse jejunum as evidenced by RT-PCR (mean, Student's t-test, *P=0.01, n=10). (D) Cav2 mRNA is decreased 75% in CAV1^IEC-KO mouse jejunum as evidenced by RT-PCR (mean, Student's t-test, *P=0.01, n=10). (E,F) CAV1 protein is reduced in the jejunum of CAV1^IEC-KO mice as measured by western blot (F) and normalized to α-tubulin. (E) Data are expressed relative to Cav1^fl/fl WT CAV1 protein, n=3 western blots, five WT and five CAV1^IEC-KO mice per blot, Student's t-test; *P<0.05. (G,H) Body mass of male (G) and female (H) mice; mice were fed a low-fat (10%) or high-fat (60%) diet starting at 10 weeks (n=10-15). HFD mice had significantly higher body mass than LFD mice, but loss of intestinal epithelial cell CAV1 did not affect body mass. Mean±s.e.m, linear regression; *P<0.05.