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. 2017 Mar 1;10(3):215–224. doi: 10.1242/dmm.028225

Fig. 6.

Fig. 6.

siRNA knockdown of CWC22 increases DRG neurite outgrowth. (A) Representative images of DRG neurons with scrambled siRNA or CWC22 siRNA. Note the increase in neurite outgrowth. Scale bar: 100 μm. (B) CWC22 siRNA significantly decreased Cwc22 mRNA in adult DRG sensory neurons. *P<0.05, unpaired one-tailed Student's t-test. (C) Neurite outgrowth (total length of candidate neurites). Scrambled: 1195±193 (mean±s.e.m.), siCWC22: 1440±182; n=7, *P=0.044, paired two-tailed Student's t-test. (D) Maximum process length (maximal length over all processes). Scrambled: 383±67, siCWC22: 452±67; n=7, P=0.059, paired two-tailed Student's t-test. (E) Number of processes (number of main neurites sprouting from the cell body). Scrambled: 5.60±0.17, siCWC22: 6.11±0.23; n=7, *P=0.011, paired two-tailed Student's t-test. (F) Number of branches (total branch points). Scrambled: 131±36, siCWC22: 168±44; n=4, P=0.2, paired two-tailed Student's t-test. (G) CWC22 siRNA was associated with a borderline increase in Smn mRNA. Scrambled: 1.03±0.14, siCWC22: 1.89±0.43; n=4, P=0.053, unpaired one-tailed Student's t-test.