Fig. 3.

Global axis patterning is retained following β1-integrin RNAi. Regenerating tail fragments were fixed at the indicated times and stained for markers of body-wide patterning of the anteroposterior axis (notum, sFRP-1), the mediolateral axis (wnt5, slit) and the dorsoventral axis (bmp4). β1-integrin RNAi did not disrupt polarized expression of notum at the anterior versus posterior pole (5/5 animals) or of sFRP-1 (8/8 animals). β1-integrin RNAi caused reduced regeneration of the pharynx and excess slit expression in that region, with a slight increase in width along the dorsal anterior midline but otherwise normal medial expression (10/10 animals). β1-integrin RNAi retained expression of wnt5 at the lateral periphery (14/14 animals), with some neurospheroid-related staining evident (6/14 animals). bmp4 expression remained dorsally restricted in β1-integrin(RNAi) animals (5/5 animals). Scale bars: 150 µm.