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. 2017 Mar 1;144(5):889–896. doi: 10.1242/dev.145672

Fig. 4.

Fig. 4.

Both Flt1 isoforms reduce transient contacts prior to connection. (A) Schematic showing Flt1 isoforms and sequences targeted by the indicated siRNAs. (B,C) Western blot of HUVEC lysates (B) and concentrated conditioned media (C) with indicated knockdowns. Control (NT), total FLT1 KD (si-Flt1), soluble FLT1 KD (si-sFlt1), membrane-tethered FLT1 KD (si-mFlt1). Fold change from control is shown beneath lanes. (D) Quantification of the number of contacts prior to connection. One-way ANOVA with Bonferroni's post-hoc correction; **P<0.01, ****P<0.0001; NS, not significant. Error bars, mean±95% CI. (E-G) Stills from DIC movies showing examples of transient contacts (E-G, red arrowheads) and stable connections (F,G, red arrows) with the indicated treatments. Time, h:min. Scale bars: 10 µm.