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Gene expresson of cytokines in BMDCs treated with lactic acid bacteria. Bone marrow-derived dendritic cells (BMDCs) were culture with heat-killed L. bulgaricus ME-552 or S. thermophilus ME-553 for six hours, and collected for RNA from BMDCs. The ratio of cells in the co-culture was as follows: CD11c+ cell:ME552 or ME553 = 1:0, 5, or 50 (multiplicity of infection—MOI: 0, 5, or 50). qRT-PCR was performed on amplified cDNA to measure gene expression of Il6, Il12p35, Il1b, and Il23p19.
