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. 2017 Feb 16;5(1):7. doi: 10.3390/microorganisms5010007

Figure 3.

Figure 3

Impact of the presence of Ca2+ on swarming and sliding mediated surface colonization of B. subtilis DK1042 and ∆hag strains, respectively, on Lysogeny broth (LB) and 2×SG medium. Swarming diameter of B. subtilis DK1042 strain after 3 to 7 h on LB (A) and 2×SG (B) media with 0.7% agar without (white bars) or with 1 (stripped bars), 10 (grey bars), 100 mM (black bars) Ca2+ supplemented. Sliding diameter of B. subtilis ∆hag strain (C) after 24 and 48 h on LB (left) and 2×SG (right) media supplemented with various amount of Ca(NO3)2 (labelling similar to S4A). Swarming (D) and sliding (E) disk of wild type (WT) and ∆hag strains, respectively, 24 h after inoculation on LB (above) and 2×SG (below) media with 0.7% agar in the absence or presence of various amounts of Ca2+ supplementation. Scale bars indicate 2 cm. Growth properties of B. subtilis DK1042 (F) in 2×SG medium supplemented with different amount Ca(NO3)2 from 1 mM to 100 mM.