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. 2017 Feb 21;5(1):8. doi: 10.3390/microorganisms5010008

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The 16S rRNA gene phylogenetic tree of Rfx. antarcticus strain ANT.BR^T and related Betaproteobacteria with Escherichia coli (Gammaproteobacteria) as the outgroup. rRNA gene sequences were obtained from the National Center for Biotechnology Information (NCBI) GenBank database [6] and aligned with ClustalW using Mega7 [7], with a final data set of 1380 nucleotides. Phylogenetic analysis was conducted using the maximum likelihood method in conjunction with the Jukes–Cantor correction [8]. Bootstrap values above 50 (100 replicates) are shown at their respective nodes.