Table 1.

Electrophoretic techniques useful for the detection of proteins in colloidal nanometric samples.

Technique	Characteristics that analyses	Advantages	Disadvantages	References
Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE)	Proteins present in a sample separated as a function of their molecular weight	Simple and affordable	Requires preparative steps	[21,25,26,27,28]
Rocket immunoelectrophoresis and radial immunodifusion	Quantification of individual proteins Study of BSA interaction with nanoparticles	Simple, quick, and reproducible High accuracy Quantitative results Use of little equipment No preparative separation steps 1	Requires a calibration curve	[10,20,21,24,29,30,31]
2D immunoelectrophoresis	Quantification of individual proteins Study of the activation of the complement system (C3 protein)	Simple, quick, and reproducible Use of little equipment Quantitative results No preparative separation steps	Requires a calibration curve Difficult to extrapole to in vivo results Low sensitivity	[24,32,33,34]